rhodamine (Vector Laboratories)

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Vector Laboratories rhodamine
Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhodamine+wheat+germ+agglutinin/Rhodamine+labeled+Wheat+Germ+Agglutinin+(WGA)/pm42831433-193-46-47
Average 96 stars, based on 271 article reviews

rhodamine - by Bioz Stars, 2026-10

96/100 stars

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Staining:

Article Title: Knockout of the Intracellular Calcium Conducting Ion Channel Mitsugumin 23 ( <scp>MG23</scp> ) Protects Against Pressure Overload Induced Left Ventricular Hypertrophy and Cardiac Dysfunction
Article Snippet: In cardiac dysfunction, intracellular Ca2+dynamics are disrupted leading to leakage of Ca2+ from the sarcoplasmic reticulum (SR).. This results in diminished cardiac contractility and impaired cardiac function.. In cardiac tissue, the underlying molecular mechanisms responsible for RyR2independent Ca2+leak are poorly understood.

Article Title: Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.
Article Snippet: .. The staining solution was added to the wells and incubated at room A TIC LE IN PR ES S temperature for 10 min. Rhodamine Wheat Germ Agglutinin (# RL-1022, Vector, USA) diluted in 1 μg/mL with HBSS was used for staining. ..

Article Title: Ciliary tip actin dynamics regulate photoreceptor outer segment integrity.
Article Snippet: In all cases, cell lysates were digested using sequential digestion of LysC (Wako) and trypsin (Pierce) using the FASP protocol (https:// pubmed.ncbi.nlm.nih.gov/25063446/). .. 8 μm cryosections were collected and stained for rhodamine wheat germ agglutinin (Vector RL-1022) and phalloidin conjugated to Atto647N (Sigma 65906) or cofilin (CST 5175) and phalloidin conjugated to Atto647N (Sigma 65906). .. The sections were then mounted in Prolong Glass (Invitrogen P36980).

Article Title: Ciliary tip actin dynamics regulate photoreceptor outer segment integrity
Article Snippet: .. 8 μm cryosections were collected and stained for rhodamine wheat germ agglutinin (Vector RL-1022) and phalloidin conjugated to Atto647N (Sigma 65906) or cofilin (CST 5175) and phalloidin conjugated to Atto647N (Sigma 65906). .. The sections were then mounted in Prolong Glass (Invitrogen P36980).

Incubation:

Whole Genome Amplification:

Western Blot:

Article Title: The AE4 transporter mediates kidney acid-base sensing
Article Snippet: .. Wagner ), rabbit anti-α-ENaC (WB 1:3000, generous gift by J. Loffing, Institute of Anatomy, University of Zurich, Switzerland ), rabbit anti-β-ENaC (WB 1:10,000, StressMarq SPC 404), rabbit anti γ-ENaC (WB 1:3000, StressMarq SPC 405), rabbit anti-NCC (WB 1:5000, Millipore AB3553), sheep anti-NCC Thr60P (WB 1:5000, MRC PPU S995B), rabbit anti-NHE3 (WB 1:10,000, StressMarq SPC 400D), mouse anti-NHE3 Ser552P (WB 1:10,000, Novus NB110-81529 Clone 14D5), rhodamine wheat germ agglutinin (IF 1:400; Vector), and Hoechst (1:1000; Molecular Probes). .. Secondary antibodies used were either horseradish peroxidase- or fluorescent dye-conjugated, affinity-purified antibodies (anti-goat: IF 1:200, Jackson ImmunoResearch Laboratories; anti-guinea pig: IF 1:200, Jackson ImmunoResearch Laboratories, anti-rabbit: IF 1:200, Jackson ImmunoResearch Laboratories, anti-rabbit: WB 1:5000 to 1:10,000, Dako Cytomation; anti-sheep WB 1:15,000, Thermo Fisher Scientific).

Article Title: The AE4 transporter mediates kidney acid-base sensing.
Article Snippet: .. Wagner29), rabbit anti-αENaC (WB 1:3000, generous gift by J. Loffing, Institute of Anatomy, University of Zurich, Switzerland30), rabbit anti-β-ENaC (WB 1:10,000, StressMarq SPC 404), rabbit anti γ-ENaC (WB 1:3000, StressMarq SPC 405), rabbit anti-NCC (WB 1:5000, Millipore AB3553), sheep anti-NCC Thr60P (WB 1:5000, MRC PPU S995B), rabbit anti-NHE3 (WB 1:10,000, StressMarq SPC 400D), mouse anti-NHE3 Ser552P (WB 1:10,000, Novus NB110-81529 Clone 14D5), rhodamine wheat germ agglutinin (IF 1:400; Vector), and Hoechst (1:1000; Molecular Probes). .. Secondary antibodies used were either horseradish peroxidase- or fluorescent dye-conjugated, affinity-purified antibodies (anti-goat: IF 1:200, Jackson ImmunoResearch Laboratories; anti-guinea pig: IF 1:200, Jackson ImmunoResearch Laboratories, anti-rabbit: IF 1:200, Jackson ImmunoResearch Laboratories, anti-rabbit: WB 1:5000 to 1:10,000, Dako Cytomation; anti-sheep WB 1:15,000, Thermo Fisher Scientific).

other:

Article Title: The proteasome modulates endocytosis specifically in glomerular cells to promote kidney filtration.
Article Snippet: Primary antibodies used for the study were: rabbit anti-ubiquitin (immunofluorescence microscopy (IF) 1:300, Novus, #NB300-129); guinea pig anti-nephrin (IF 1:200, Origene, #BP5030); rabbit anti-β5c (IF 1:300, WB 1:5000, laboratory stock X. Wang, University of South Dakota, USA); rabbit anti-α2 (WB 1:1000, Cell Signaling, #2455); rabbit anti-α-actinin-4 (WB 1:1000, Immunoglobe, #0042-05); rabbit anti-p62 (WB 1:2000, Sigma-Aldrich, #P0067), guinea pig anti-p62 (IF 1:500, ProGene, #GP62-c); goat anti-Mrc2 (WB 1:1000, IF 1:200 R&D Systems, #AF4789); AF488 mouse anti H2Kd-(FACS-sort 1:250, BioLegend, Clone #SF1-1.1); BV421 rat anti-CD31 (FACS-sort 1:800, BD Horizon, #562939); AF647 rat anti-CD73 (FACS-sort 1:2000, BioLegend, #127208); AF700 rat anti-CD73 (FACS-sort 1:2000, BioLegend, #127230); PE hamster anti-podoplanin (FACS-sort 1:200, BioLegend, #127408); AF700 rat anti-CD45 (FACS-sort 1:100, BioLegend, #103128); APC-Cy7 rat anti-CD45 (FACS-sort 1:100, BioLegend, #103116); FITC mouse anti-pig CD31 (IF 1:30, Bio-Rad, #MCA1746F); goat anti-biotin (WB 1:1000, Thermo Fisher, #PA1-26792); AF546 rat anti-endomucin (IF 1:50, Santa Cruz, #sc-65495); rabbit anti-K48pUb (IF 1:300, WB 1:1000, Abcam, #ab140601); rabbit anti-LC3B (WB 1:5000, IF 1:50, Cell Signaling, #3868S) rabbit anti-Limp2 (WB 1:500, IF 1:1000, laboratory stock P. Saftig, CAU Kiel, Germany), rabbit anti-Lamp2 (WB 1:1000, IF 1:300, Sigma-Aldrich, #L0668), mouse anti-human LAMP1 (IF 1:200, DSHB, # H4A3); rabbit anti-p57 (IF 1:400, Santa Cruz, #sc-8298); rhodamine-wheat germ agglutinin (IF 1:400, WGA, Vector, #RL-1022); DyLight649-lycopersicon esculentum (tomato) lectin (i.v. injection of 10ml of a 40 μg/ml solution, Vector, #DL-1178-1); DyLight488Phalloidin (IF 1:200, Molecular Probes, #21833); AF488 donkey antirabbit IgG (IF 1:200, Cy2, WB 1:1000, Jackson ImmunoResearch, #711- 545-152); Cy5 donkey anti-mouse IgG (IF 1:200, Cy5, Jackson ImmunoResearch, #715-175-151); guinea pig anti-synaptopodin (IF 1:400, Synaptic Systems, #163004); goat anti-collagen 4 (IF 1:400, Southern Biotechnologies, #1340-01); mouse anti-Rpt5 (WB 1:1000, Enzo, #BMLPW8770); rabbit anti-Lmp7 ((β5i) WB 1:5000, laboratory stock E. Krüger, IF 1:300); mouse anti-β-actin (WB 1:10,000, Sigma-Aldrich, #A5441); goat anti-cathepsin D (WB 1:100, Santa Cruz, #sc-6486); rabbit anti-calreticulin (WB 1:500, Abcam, #ab92516).

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